The Lizard Locomotion Analysis project aims to develop a comprehensive understanding of lizard running behavior through advanced computer vision techniques, specifically utilizing DeepLabCut and SLEAP for precise pose estimation and movement analysis. By creating detailed anatomical landmarks across the lizard’s body, this project will enable quantitative analysis of various locomotor patterns and behavioral characteristics, moving beyond traditional center-of-mass tracking to capture complex biomechanical interactions during movement. Through this open-source approach, we will document multiple behavioral patterns across different body segments, contributing to abroader understanding of lizard locomotion while establishing standardized protocols for future research in animal biomechanics. This work will not only advance our understanding of lizard movement patterns but also contribute to the growing field of automated behavioral analysis in biological research.