Lokugamage, M. P., Gan, Z., Zurla, C., Levin, J., Islam, F. Z., Kalathoor, S., Sato, M., Sago, C. D., Santangelo, P. J. & Dahlman, J. E. Mild Innate Immune Activation Overrides Efficient Nanoparticle-Mediated RNA Delivery. Adv Mater 32, e1904905 (2020).
Toy, R., Pradhan, P., Ramesh, V., Di Paolo, N. C., Lash, B., Liu, J., Blanchard, E. L., Pinelli, C. J., Santangelo, P. J., Shayakhmetov, D. M. & Roy, K. Modification of primary amines to higher order amines reduces in vivo hematological and immunotoxicity of cationic nanocarriers through TLR4 and complement pathways. Biomaterials 225, 119512 (2019).
Blanchard, E. L., Argyropoulou, D., Zurla, C., Bhosle, S. M., Vanover, D. & Santangelo, P. J. Quantification and Localization of Protein-RNA Interactions in Patient-Derived Archival Tumor Tissue. Cancer Res 79, 5418–5431 (2019).
Lindsay, K. E., Bhosle, S. M., Zurla, C., Beyersdorf, J., Rogers, K. A., Vanover, D., Xiao, P., Arainga, M., Shirreff, L. M., Pitard, B., Baumhof, P., Villinger, F. & Santangelo, P. J. Visualization of early events in mRNA vaccine delivery in non-human primates via. Nat Biomed Eng 3, 371–380 (2019).
Blanchard, E. L., Loomis, K. H., Bhosle, S. M., Vanover, D., Baumhof, P., Pitard, B., Zurla, C. & Santangelo, P. J. Proximity Ligation Assays for In Situ Detection of Innate Immune Activation: Focus on In Vitro-Transcribed mRNA. Mol Ther Nucleic Acids 14, 52–66 (2019).
Xu, Y., Huang, L., Kirschman, J. L., Vanover, D. A., Tiwari, P. M., Santangelo, P. J., Shen, X. & Russell, D. G. Exploitation of Synthetic mRNA To Drive Immune Effector Cell Recruitment and Functional Reprogramming In Vivo. J Immunol 202, 608–617 (2019).
Sago, C. D., Lokugamage, M. P., Paunovska, K., Vanover, D. A., Monaco, C. M., Shah, N. N., Gamboa Castro, M., Anderson, S. E., Rudoltz, T. G., Lando, G. N., Munnilal Tiwari, P., Kirschman, J. L., Willett, N., Jang, Y. C., Santangelo, P. J., Bryksin, A. V. & Dahlman, J. E. High-throughput in vivo screen of functional mRNA delivery identifies nanoparticles for endothelial cell gene editing. Proc Natl Acad Sci U S A 115, E9944–E9952 (2018).
Chambers, D. M., Moretti, L., Zhang, J. J., Cooper, S. W., Chambers, D. M., Santangelo, P. J. & Barker, T. H. LEM domain-containing protein 3 antagonizes TGFbeta-SMAD2/3 signaling in a stiffness-dependent manner in both the nucleus and cytosol. J Biol Chem 293, 15867–15886 (2018).
Tiwari, P. M., Vanover, D., Lindsay, K. E., Bawage, S. S., Kirschman, J. L., Bhosle, S., Lifland, A. W., Zurla, C. & Santangelo, P. J. Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection. Nat Commun 9, 3999 (2018).
Loomis, K. H., Lindsay, K. E., Zurla, C., Bhosle, S. M., Vanover, D. A., Blanchard, E. L., Kirschman, J. L., Bellamkonda, R. V. & Santangelo, P. J. In Vitro Transcribed mRNA Vaccines with Programmable Stimulation of Innate Immunity. Bioconjug Chem 29, 3072–3083 (2018).
Lertjuthaporn, S., Cicala, C., Van Ryk, D., Liu, M., Yolitz, J., Wei, D., Nawaz, F., Doyle, A., Horowitch, B., Park, C., Lu, S., Lou, Y., Wang, S., Pan, R., Jiang, X., Villinger, F., Byrareddy, S. N., Santangelo, P. J., Morris, L., Wibmer, C. K., Biris, K., Mason, R. D., Gorman, J., Hiatt, J., Martinelli, E., Roederer, M., Fujikawa, D., Gorini, G., Franchini, G., Arakelyan, A., Ansari, A. A., Pattanapanyasat, K., Kong, X.-P., Fauci, A. S. & Arthos, J. Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to alpha4beta7. PLoS Pathog 14, e1007278 (2018).
Santangelo, P. J., Cicala, C., Byrareddy, S. N., Ortiz, K. T., Little, D., Lindsay, K. E., Gumber, S., Hong, J. J., Jelicic, K., Rogers, K. A., Zurla, C., Villinger, F., Ansari, A. A., Fauci, A. S. & Arthos, J. Early treatment of SIV+ macaques with an alpha4beta7 mAb alters virus distribution and preserves CD4(+) T cells in later stages of infection. Mucosal Immunol 11, 932–946 (2018).
Arthos, J., Cicala, C., Nawaz, F., Byrareddy, S. N., Villinger, F., Santangelo, P. J., Ansari, A. A. & Fauci, A. S. The Role of Integrin alpha4beta7 in HIV Pathogenesis and Treatment. Curr HIV/AIDS Rep 15, 127–135 (2018).
Paunovska, K., Sago, C. D., Monaco, C. M., Hudson, W. H., Castro, M. G., Rudoltz, T. G., Kalathoor, S., Vanover, D. A., Santangelo, P. J., Ahmed, R., Bryksin, A. V. & Dahlman, J. E. A Direct Comparison of in Vitro and in Vivo Nucleic Acid Delivery Mediated by Hundreds of Nanoparticles Reveals a Weak Correlation. Nano Lett 18, 2148–2157 (2018).
Bhosle, S. M., Loomis, K. H., Kirschman, J. L., Blanchard, E. L., Vanover, D. A., Zurla, C., Habrant, D., Edwards, D., Baumhof, P., Pitard, B. & Santangelo, P. J. Unifying in vitro and in vivo IVT mRNA expression discrepancies in skeletal muscle via mechanotransduction. Biomaterials 159, 189–203 (2018).
Vanover, D., Smith, D. V., Blanchard, E. L., Alonas, E., Kirschman, J. L., Lifland, A. W., Zurla, C. & Santangelo, P. J. RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane. Nat Commun 8, 667 (2017).
Kirschman, J. L., Bhosle, S., Vanover, D., Blanchard, E. L., Loomis, K. H., Zurla, C., Murray, K., Lam, B. C. & Santangelo, P. J. Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and. Nucleic Acids Res 45, e113 (2017).
Udhayakumar, V. K., De Beuckelaer, A., McCaffrey, J., McCrudden, C. M., Kirschman, J. L., Vanover, D., Van Hoecke, L., Roose, K., Deswarte, K., De Geest, B. G., Lienenklaus, S., Santangelo, P. J., Grooten, J., McCarthy, H. O. & De Koker, S. Arginine-Rich Peptide-Based mRNA Nanocomplexes Efficiently Instigate Cytotoxic T Cell Immunity Dependent on the Amphipathic Organization of the Peptide. Adv Healthc Mater 6, (2017).
Hampton, C. M., Strauss, J. D., Ke, Z., Dillard, R. S., Hammonds, J. E., Alonas, E., Desai, T. M., Marin, M., Storms, R. E., Leon, F., Melikyan, G. B., Santangelo, P. J., Spearman, P. W. & Wright, E. R. Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells. Nat Protoc 12, 150–167 (2017).
Zurla, C., Jung, J., Blanchard, E. L. & Santangelo, P. J. A Novel Method to Quantify RNA-Protein Interactions In Situ Using FMTRIP and Proximity Ligation. Methods Mol Biol 1468, 155–170 (2017).
Byrareddy, S. N., Arthos, J., Cicala, C., Villinger, F., Ortiz, K. T., Little, D., Sidell, N., Kane, M. A., Yu, J., Jones, J. W., Santangelo, P. J., Zurla, C., McKinnon, L. R., Arnold, K. B., Woody, C. E., Walter, L., Roos, C., Noll, A., Van Ryk, D., Jelicic, K., Cimbro, R., Gumber, S., Reid, M. D., Adsay, V., Amancha, P. K., Mayne, A. E., Parslow, T. G., Fauci, A. S. & Ansari, A. A. Sustained virologic control in SIV+ macaques after antiretroviral and alpha4beta7 antibody therapy. Science 354, 197–202 (2016).
Liu, Y., Yan, J., Santangelo, P. J. & Prausnitz, M. R. DNA uptake, intracellular trafficking and gene transfection after ultrasound exposure. J Control Release 234, 1–9 (2016).
Alonas, E., Vanover, D., Blanchard, E., Zurla, C. & Santangelo, P. J. Imaging viral RNA using multiply labeled tetravalent RNA imaging probes in live cells. Methods 98, 91–98 (2016).
Groves, B., Chen, Y.-J., Zurla, C., Pochekailov, S., Kirschman, J. L., Santangelo, P. J. & Seelig, G. Computing in mammalian cells with nucleic acid strand exchange. Nat Nanotechnol 11, 287–294 (2016).
Zurla, C., Jung, J. & Santangelo, P. J. Can we observe changes in mRNA ‘state’? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes. Analyst 141, 548–562 (2016).
Yang, X., Xie, H., Alonas, E., Liu, Y., Chen, X., Santangelo, P. J., Ren, Q., Xi, P. & Jin, D. Mirror-enhanced super-resolution microscopy. Light Sci Appl 5, (2016).
Yi, H., Strauss, J. D., Ke, Z., Alonas, E., Dillard, R. S., Hampton, C. M., Lamb, K. M., Hammonds, J. E., Santangelo, P. J., Spearman, P. W. & Wright, E. R. Native immunogold labeling of cell surface proteins and viral glycoproteins for cryo-electron microscopy and cryo-electron tomography applications. J Histochem Cytochem 63, 780–792 (2015).
Santangelo, P. J., Rogers, K. A., Zurla, C., Blanchard, E. L., Gumber, S., Strait, K., Connor-Stroud, F., Schuster, D. M., Amancha, P. K., Hong, J. J., Byrareddy, S. N., Hoxie, J. A., Vidakovic, B., Ansari, A. A., Hunter, E. & Villinger, F. Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques. Nat Methods 12, 427–432 (2015).
Roller, B. T., Munson, J. M., Brahma, B., Santangelo, P. J., Pai, S. B. & Bellamkonda, R. V. Evans blue nanocarriers visually demarcate margins of invasive gliomas. Drug Deliv Transl Res 5, 116–124 (2015).
Wigington, C. P., Jung, J., Rye, E. A., Belauret, S. L., Philpot, A. M., Feng, Y., Santangelo, P. J. & Corbett, A. H. Post-transcriptional regulation of programmed cell death 4 (PDCD4) mRNA by the. J Biol Chem 290, 3468–3487 (2015).
Byrareddy, S. N., Kallam, B., Arthos, J., Cicala, C., Nawaz, F., Hiatt, J., Kersh, E. N., McNicholl, J. M., Hanson, D., Reimann, K. A., Brameier, M., Walter, L., Rogers, K., Mayne, A. E., Dunbar, P., Villinger, T., Little, D., Parslow, T. G., Santangelo, P. J., Villinger, F., Fauci, A. S. & Ansari, A. A. Targeting alpha4beta7 integrin reduces mucosal transmission of simian immunodeficiency virus and protects gut-associated lymphoid tissue from infection. Nat Med 20, 1397–1400 (2014).
Kiss, G., Holl, J. M., Williams, G. M., Alonas, E., Vanover, D., Lifland, A. W., Gudheti, M., Guerrero-Ferreira, R. C., Nair, V., Yi, H., Graham, B. S., Santangelo, P. J. & Wright, E. R. Structural analysis of respiratory syncytial virus reveals the position of M2-1 between the matrix protein and the ribonucleoprotein complex. J Virol 88, 7602–7617 (2014).
Alonas, E., Lifland, A. W., Gudheti, M., Vanover, D., Jung, J., Zurla, C., Kirschman, J., Fiore, V. F., Douglas, A., Barker, T. H., Yi, H., Wright, E. R., Crowe, J. E. J. & Santangelo, P. J. Combining single RNA sensitive probes with subdiffraction-limited and live-cell imaging enables the characterization of virus dynamics in cells. ACS Nano 8, 302–315 (2014).
Xie, H., Liu, Y., Jin, D., Santangelo, P. J. & Xi, P. Analytical description of high-aperture STED resolution with 0-2pi vortex phase modulation. J Opt Soc Am A Opt Image Sci Vis 30, 1640–1645 (2013).
Jung, J., Lifland, A. W., Zurla, C., Alonas, E. J. & Santangelo, P. J. Quantifying RNA-protein interactions in situ using modified-MTRIPs and proximity ligation. Nucleic Acids Res 41, e12 (2013).
Jung, J., Lifland, A. W., Alonas, E. J., Zurla, C. & Santangelo, P. J. Characterization of mRNA-cytoskeleton interactions in situ using FMTRIP and proximity ligation. PLoS One 8, e74598 (2013).
Lifland, A. W., Jung, J., Alonas, E., Zurla, C., Crowe, J. E. J. & Santangelo, P. J. Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. J Virol 86, 8245–8258 (2012).
Shaikh, F. Y., Cox, R. G., Lifland, A. W., Hotard, A. L., Williams, J. V., Moore, M. L., Santangelo, P. J. & Crowe, J. E. J. A critical phenylalanine residue in the respiratory syncytial virus fusion protein cytoplasmic tail mediates assembly of internal viral proteins into viral filaments and particles. mBio 3, (2012).
Santangelo, P. J., Alonas, E., Jung, J., Lifland, A. W. & Zurla, C. Probes for intracellular RNA imaging in live cells. Methods Enzymol 505, 383–399 (2012).
Liu, Y., Ding, Y., Alonas, E., Zhao, W., Santangelo, P. J., Jin, D., Piper, J. A., Teng, J., Ren, Q. & Xi, P. Achieving lambda/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. PLoS One 7, e40003 (2012).
Lifland, A. W., Zurla, C., Yu, J. & Santangelo, P. J. Dynamics of native beta-actin mRNA transport in the cytoplasm. Traffic 12, 1000–1011 (2011).
Zurla, C., Lifland, A. W. & Santangelo, P. J. Characterizing mRNA interactions with RNA granules during translation initiation inhibition. PLoS One 6, e19727 (2011).
Lindquist, M. E., Lifland, A. W., Utley, T. J., Santangelo, P. J. & Crowe, J. E. J. Respiratory syncytial virus induces host RNA stress granules to facilitate viral replication. J Virol 84, 12274–12284 (2010).
Lifland, A. W., Zurla, C. & Santangelo, P. J. Single molecule sensitive multivalent polyethylene glycol probes for RNA imaging. Bioconjug Chem 21, 483–488 (2010).
Santangelo, P. J. Molecular beacons and related probes for intracellular RNA imaging. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2, 11–19 (2010).
Santangelo, P. J., Lifland, A. W., Curt, P., Sasaki, Y., Bassell, G. J., Lindquist, M. E. & Crowe, J. E. J. Single molecule-sensitive probes for imaging RNA in live cells. Nat Methods 6, 347–349 (2009).