Kristof, A., Allen, C., Mizote, P., Briggs, S., Jian, Z., Nash, P., and Blazeck, J. “Engineering Novel CRISPRi Repressors for Highly Efficient Mammalian Gene Regulation.” Under revision.
Haikal, Y. and Blazeck, J. (2024) “Exploiting protein domain modularity to enable synthetic control of engineered cells.” Current Opinion in Biomedical Engineering. 31: 100550. Invited submission
Haikal, Y., Nguyen, J., and Blazeck, J. “Determining the Serum Stability of Human Adenosine Deaminase 1 Enzyme.” Journal of Visualized Experiments. Accepted. – Invited submission
Cox, J.R., Fox, A., Lenehan, C., Pivnik, L., Manion, M., and Blazeck, J. (2024) “Engineering CREB-activated promoters for adenosine-induced gene expression.” Biotechnology Journal. 19:2300446.
Jennings, M.R., Min, Xu, G.S., Homayuni, K., Suresh, B., Haikal, Y.A., and Blazeck, J. (2024) “Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant.” Protein Expression and Purification. 213:106362.
Cazier, A.P., Irvin, O.M., Chávez, L.S., Dalvi, S., Abraham, H., Wickramanayake, N., Yellayi, S., and Blazeck J. (2023) “A rapid antibody enhancement platform in Saccharomyces cerevisiae using an improved, diversifying CRISPR base editor.” ACS Synthetic Biology. 12(11):3287–3300.
Cox, J.R., Jennings, M.R., Lenehan, C., Manion, M., Courville, S. and Blazeck, J. (2023) “Rational engineering of an improved adenosine deaminase 2 enzyme for weaponizing T-cell therapies.” Immuno-Oncology and Technology. 19:100394.
Holland, K. and Blazeck, J. (2022) “High throughput mutagenesis and screening for yeast engineering“. Journal of Biological Engineering. 16(1):1-12.
Blazeck, J.#, Karamitros, C.S.#, et. al. “Bypassing evolutionary dead-ends and switching the rate-limiting step of a human immunotherapeutic enzyme.” Nature Catalysis. 5:952–967
Ma, M.T&., Jennings, M.R.&, Blazeck, J.#, and Lieberman, R#. (2022) “Catalytically active holo Homo sapiens adenosine deaminase I adopts a closed conformation.” Acta Crystallographica Section D: Structural Biology. 78(1):91-103.
Jennings, M.R., Munn, D., and Blazeck, J. (2021) “Immunosuppressive metabolites in tumoral immune evasion: redundancies, clinical efforts, and pathways forward.” Journal for Immunotherapy of Cancer. 9(10):e003013
Cox, J.R., and Blazeck, J. (2021) “Protein Engineering: A Driving Force Toward Synthetic Immunology.” Trends in Biotechnology. 40(4):509-521.
Cazier, A., and Blazeck, J. (2021). “Advances in promoter engineering: Novel applications and predefined transcriptional control.” Biotechnology Journal. 16(10):2100239.
Lindesmith, L., McDaniel, J., Changela, A., Verardi, R., Kerr, S., Costantini, V., Brewer-Jensen, P. , Mallory, M., Voss, W., Boutz, D., Blazeck, J., Ippolito, G., Vinje, J., Kwong, P., Georgiou, G., and Baric, R. (2019) “Sera antibody repertoire analyses reveal mechanisms of broad and pandemic strain neutralizing responses after human norovirus vaccination.” Immunity. 50(6):1530-1541.
Triplett, T*., Garrison, K*., Marshall, N*., Donkor, M*., Blazeck, J., Lamb, C., Qerqez, A., Tano, Y., Lu, W.-C., Karamitros, C., Ford, K., Tan, B., Zhang, M., Kumada, Y., Yemeny, M.S., Sentandreu, E., Fromm, G., Tiziani, S., Schreiber, T. H., Manfredi, M., Ehrlich, L. I. R., Stone, E., and Georgiou, G. (2018) “Reversal of indoleamine 2,3-dioxygenase–mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme.” Nature Biotechnology, 36:758–764.
Blazeck, J., Hill, A., Jamoussi, M., Pan, A., Miller, J., Alper, H. S. (2015) “Metabolic engineering of Yarrowia lipolytica for itaconic acid production.” Metabolic Engineering. 32:66-73.
Liu, L., Markham, K., Blazeck, J., Zhou, N., Leon, D., Otoupal, P., Alper, H. S. (2015) “Surveying the lipogenesis landscape in Yarrowia lipolytica through understanding the function of a Mga2p regulatory protein mutant.” Metabolic Engineering. 31:102-111.
Blazeck, J., Miller, J., Pan, A., Gengler, J., Holden C., Alper, H. S. (2014) “Metabolic Engineering of Saccharomyces cerevisiae for itaconic acid production.” Applied Microbiology and Biotechnology. 98:8155-8164.
Blazeck, J.*, Hill, A.*, Liu, L.*, Knight, R. Miller, J., Pan, A., Otoupal, P., Alper, H. S. (2014) “Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production.” Nature Communications. 5(3131) doi:10.1038/ncomms4131.
Blazeck, J., Liu, L., Knight, R., Alper, H. S. (2013) “Heterologous production of pentane in the oleaginous yeast Yarrowia lipolytica.” Journal of Biotechnology. 165(3-4): 184-194.
Blazeck, J., Reed, B., Garg, R., Gerstner, R., Pan, A., Agarwala, V., Alper, H. S. (2013). “Generalizing a Hybrid Synthetic Promoter Approach in Yarrowia lipolytica.” Applied Microbiology and Biotechnology. 97(7): 3037-3052.
Blazeck, J. and Alper, H. (2013). “Promoter Engineering: Recent Advances in Controlling Transcription at the Most Fundamental Level.” Biotechnology Journal. 8(1): 46-58.
Blazeck, J., Garg, R., Reed, B., Alper, H. S. (2012). “Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.” Biotechnology and Bioengineering. 109(11) 2884-2995.
Lanza, A., Blazeck, J., Crook, N. Alper, H. S. (2012). “Linking Yeast Gcn5p Catalytic Function and Gene Regulation Using a Quantitative, Graded Dominant Mutant Approach.” PLoS ONE. 7(4): e36193.
Reed, B., Blazeck, J., Alper, H. S. (2012). “Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes.” Journal of Biotechnology. 158(3): 75-79.
Blazeck, J.*, Liu, L.*, Redden, H., Alper, H. S. (2011). “Tuning Gene Expression in Yarrowia lipolytica by a Hybrid Promoter Approach.” Applied and Environmental Microbiology. 77(22): 7905-7914.
Blazeck, J. and Alper, H. (2010). “Uncovering latent xylose utilization potential in Saccharomyces cerevisiae.” Biofuels. 1(5): 681-684.
Blazeck, J. and Alper, H. (2010). “Systems metabolic engineering: Genome-scale models and beyond.” Biotechnology Journal. 5(7): 647-649.
Patents
Human kynureninase enzymes and uses thereof
George Georgiou, Everett Stone, John Blazeck, Christos Karamitros
U.S. Patent No. 11,648,272 B2
Administration of kynurenine depleting enzymes for tumor therapy.
U.S. Patent No. 9,808,486 B2
George Georgiou, Everett Stone, Nicholas Marshall, John Blazeck, Wei-cheng Lu.
Composition and methods for fungal lipid production.
U.S. Patent No. 9,896,691.
Hal Alper, John Blazeck, Leqian Liu, Andrew Hill.