Background and Inter-laboratory Screening
One challenge in the field of metabolomics using mass spectrometry is inter-laboratory variability. This can lead to inaccurate assignment of features and complicated issues downstream. To combat this, we have begun a collaborative study involving 6 institutions’ laboratories (Georgia Institute of Technology, University of Michigan, University of Georgia, University of California- Davis, The Jackson Laboratory, and the Pacific Northwest National Laboratory) that analyzes 94 internal standards encompassing major compound classes in one solution for quantitation/retention time alignment for human plasma and other sample types. All analyses were conducted via liquid chromatograph mass spectrometry using reverse phase columns and Hydrophilic Interaction Chromatography (HILIC) columns. The processing and comparison of data is currently being performed here at Georgia Tech in the Fernandez group, and this is the platform for a larger study in the future.
(1) Izumi, Y.; Matsuda, F.; Hirayama, A.; Ikeda, K.; Kita, Y.; Horie, K.; Saigusa, D.; Saito, K.; Sawada, Y.; Nakanishi, H.; Okahashi, N.; Takahashi, M.; Nakao, M.; Hata, K.; Hoshi, Y.; Morihara, M.; Tanabe, K.; Bamba, T.; Oda, Y. Inter-Laboratory Comparison of Metabolite Measurements for Metabolomics Data Integration. Metabolites 2019, 9 (11), 257. https://doi.org/10.3390/metabo9110257.