How to measure H1 T1 in organic solvents:

  1. Run 1D PROTON first, and find proper o1, sw, and p1 by pulsecal.
  2. load PROTONT1 parameter folder, besides using most of the default parameters, update p1, o1, sw,
  3. try to use default d1=10s, ns=16, vdlist: 0.01, 0.05, 0.1, 0.2, 0.5, 1, 3, 5, 8, 10s; and set TD1 as the total number of the delay time points in vdlist (10 here).

How to measure C13 T1 in organic solvents:

  1. Run 1D C13CPD first, and find proper o1, sw, and p1.
  2. Load C13_T1IRIG2D_hw parameter folder (with proton decoupling), update proton o2, p2 here ready too. Double check C13’s p1, plw1, etc. are correct.
  3. try to use d1=60s, ns=8, vdlist can be: 0.5, 1, 3, 6, 10, 16, 24, 50s; and set TD1 as the total number of the delay time points in vdlist (8 here).

Process with T1T2 Application:

  1. load “2D” data, rser 1 ↙, efp↙, apk↙, absn↙ .ph, nD save (important!), and save & exit,
  2. load “2D” data again, xf2↙, abs2↙ (may try absg = 1 or 5 and see different effect, — degree of polynomial for abs, [0..5] (F2,F1))
  3. Applications → Dynamics → T1T2 → FID →Spectrum → 1
  4. Peaks/Ranges → Manual integration → define Ranges → integrate regions (choose more peaks…) → export to relaxation module and.ret.
  5. Go to Relaxation tab → check parameters (make sure to choose: 1000, -1000 for baseline correction limits, etc.) → press “>” icon to fitting, click full spectrum button, click sq; and then press “>>>” icon to fit all other points, then click “show report”, save into a text file.

Hongwei edited on 5/2/2023