| 1L | |
| Na2HPO4 | 6.8g |
| KH2PO4 | 3.0g |
| NaCl | 0.5g |
| Glucose (or D-glucose, U-13C6, 99%, ~$150/g) | 2.0g |
| NH4Cl (or 15NH4Cl, 99%, ~$30/g) | 1.0g |
| 1M MgSO4 | 1.0ml |
| 2M CaCl2 | 50ul |
| 0.1M FeCl3 | 33ul |
| 100X MEM | 10ml |
| Antibiotics (Amp or Kan) | Add just before start cell culture |
Notes:
1. Add water and other components first, finally add CaCl2 gradually with stirring, otherwise, precipitation happens!
2. For 1L M9 medium, just pass through 0.22 µm filter, don’t need autoclave.
3. MEM vitamins solution (100x) can be ordered from Fisher Scientific, catalog#: 11120052, it’s a 100ml yellow color solution. (Aliquot the 100ml into 10x 10ml and freeze them under -20°C after receive.)
Hongwei edited on 9/1/2023